Original video: https://youtu.be/HaBzBZWamvE
Time lapse movie of RDP1RDP1 (left) and rdp1 3rdp1 3 (right) Arabidopsis plants. Photos were taken every hour from 10h00 to 19h00 for 39 days under the long-day condition.
The CRISPR/Cas9-generated frameshift rdp1-3 mutant in the Col-0 background produced about half the number of pollen grains compared with the wild-type counterpart. We further observed the section of the developing anther and confirmed that the number of microspores was already decreased in the mutant at the anther stage 8. It was reported that the frameshift rdp1-3 mutants exhibited other pleiotropic phenotypes, such as reduced ovule number and delayed growth, in contrast with the effect of natural variants. Here, we found that the bolting and flowering times were significantly delayed. These results support the notion that RDP1 functions as an Mrt4 homolog in A. thaliana, in accordance with the slower growth observed in the Mrt4 null mutant in yeast.
Citation: Kakui H, Tsuchimatsu T, Yamazaki M, Hatakeyama M and Shimizu KK (2022) Pollen Number and Ribosome Gene Expression Altered in a Genome-Editing Mutant of REDUCED POLLEN NUMBER1 Gene. Front. Plant Sci. 12:768584. doi: https://doi.org/10.3389/fpls.2021.768584
Keywords: pollen number, REDUCED POLLEN NUMBER1, complementation test, CRISPR/Cas9, transcriptome, ribosomal protein
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