Original video: https://youtu.be/XSt0tCroy8U
Nematode migration, feeding site formation, withdrawal of plant assimilates, and activation of plant defence responses have a significant impact on plant growth and development. Plants display intraspecific variation in tolerance limits for root-feeding nematodes. Although disease tolerance has been recognized as a distinct trait in biotic interactions of mainly crops, we lack mechanistic insights. Progress is hampered by difficulties in quantification and laborious screening methods. We turned to the model plant Arabidopsis thaliana, since it offers extensive resources to study the molecular and cellular mechanisms underlying nematode–plant interactions. Through imaging of tolerance-related parameters, the green canopy area was identified as an accessible and robust measure for assessing damage due to cyst nematode infection. Subsequently, a high-throughput phenotyping platform simultaneously measuring the green canopy area growth of 960 A. thaliana plants was developed. This platform can accurately measure cyst nematode and root-knot nematode tolerance limits in A. thaliana through classical modelling approaches. Furthermore, real-time monitoring provided data for a novel view of tolerance, identifying a compensatory growth response. These findings show that our phenotyping platform will enable a new mechanistic understanding of tolerance to below-ground biotic stress. This time-lapse video captures the dynamic growth of Arabidopsis thaliana (Col-0) as it responds to infection by the plant-parasitic nematode H. schachtii for a period of 3 weeks. Watch the development of the plant over time, showcasing root morphology changes, infection site formation, and host-pathogen interactions.
Keywords: Arabidopsis thaliana, biotic stress, growth rate analysis, Heterodera schachtii, high-throughput phenotyping, Meloidogyne incognita, root-parasitic nematodes, tolerance
Citation: Jaap-Jan Willig, Devon Sonneveld, Joris J M van Steenbrugge, Laurens Deurhof, Casper C van Schaik, Misghina G Teklu, Aska Goverse, Jose L Lozano-Torres, Geert Smant, Mark G Sterken, From root to shoot: quantifying nematode tolerance in Arabidopsis thaliana by high-throughput phenotyping of plant development, Journal of Experimental Botany, Volume 74, Issue 18, 29 September 2023, Pages 5487–5499, https://doi.org/10.1093/jxb/erad266
Published on: 11 July 2023
Attribution 4.0 International — CC BY 4.0 - Creative Commons
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