Original video: https://youtu.be/k3TgnY3gBVU
Delivered on: 23 May 2024
This video is the second part of a two-part series on using the Li-6400XT Portable Photosynthesis System to measure CO2 response curves and calculate A-Ci curves in plants. In this part, we will focus on the following:
Setting up the Li-6400XT: We will discuss how to prepare the Li-6400XT for a CO2 response curve measurement, including selecting and cleaning a leaf, setting the environmental conditions, and opening the stomata.
Measuring the CO2 response curve: We will demonstrate how to measure the CO2 response curve of a leaf.
Analyzing the CO2 response curve: We will explain how to extract the parameters of the CO2 response curve, such as the maximum photosynthetic rate and the carboxylation efficiency.
Interpreting the A-Ci curve: We will discuss how to interpret the A-Ci curve.
Watch Part 1 here: https://youtu.be/L7sGyCH3Cb8
Video Timestamps:
0:00:00 Machine prep
0:02:50 Selecting and clean the leaf
0:06:20 Setting the environmental condition and open log file
0:10:00 Setting to open stomata and waiting it to stable
0:31:09 Setting CO2 reponse curve
0:41:18 CO2 biphasic response curve
0:51:50 Extracting the parameter from raw data and convert to the unit for ACi curve fitting tools
1:05:19 Fitting the data to ACi curve fitting tools
1:10:00 Parameters and symbols in ACi Curve fitting tools
1:22:00 Extract other data from the curve
Keywords: Li-6400XT, CO2 response curve, A-Ci curve, photosynthesis, plant physiology, gas exchange, stomatal conductance, mesophyll conductance, carboxylation efficiency, CO2 assimilation, light response curve.
Location:
Headquarter MPOB, Bangi
6, Persiaran Institut, Kawasan Institusi Bangi, 43000 Bandar Baru Bangi, Selangor
XP9R+9X Bandar Baru Bangi, Selangor
2.968770958080534, 101.7435232860253
Attribution 4.0 International — CC BY 4.0 - Creative Commons
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