Original video: https://youtu.be/qkQjcWeNWQA
Delivered on: 21 May 2024
This video provides a comprehensive guide to the LI-6400XT Portable Photosynthesis System, a powerful tool used in plant physiology research. 🌿 Through hands-on demonstrations and clear explanations, we'll walk you through the system's components, setup, operation, and troubleshooting.
What you'll learn:
LI-6400XT Overview: We'll introduce the LI-6400XT and its capabilities for measuring gas exchange in plants, providing insights into photosynthesis, respiration, and transpiration.
System Components: Get a detailed look at the console, leaf chamber, light source, and control modules, and understand their functions.
Setting Environmental Conditions: Learn how to configure the LI-6400XT to control environmental settings.
Leaf Chamber Techniques: Master the techniques for clamping leaves, opening stomata, and preventing leaks during measurements.
IRGA Calibration and Zeroing: We'll demonstrate how to calibrate and zero the infrared gas analyzer (IRGA) for accurate measurements.
Troubleshooting: Learn how to identify and resolve common issues that may arise during LI-6400XT operation.
Data Logging and A-Ci Curves: Understand how to log data and generate CO2 response curves (A-Ci curves) to analyze photosynthetic performance.
Video Timestamps:
0:00:00 Introduction
0:01:20 LI-6400 Part and Component
0:12:31 Function keys and setting of the environmental conditions
0:20:34 Main Screen
0:22:09 New measurement screen
0:23:46 Preparation checklist LI-6400, warm up
0:27:20 Summary of symbol and displays of group
0:28:18 During warm up
0:40:50 Practical setting the environmental conditions
0:46:00 Clamping the leaf
0:47:40 How to open the leaf stomata
0:49:45 Adding water to soda lime
0:54:36 Disposal and replacing the soda lime and Drierite
0:57:00 Calibrating and zeroing IRGA
1:00:00 Troubleshooting LI-6400
1:15:37 Stomata ratio in the LI-6400
1:27:00 Overview on state measurement
1:28:00 Temperature setting
1:29:45 Matching IRGA
1:31:19 Open log file
1:32:30 Clamp on leaf and condition on logging data
1:35:40 CO2 response curve/A-Ci Curve
1:43:30 Q&A
1:45:10 Selecting frond for measurement
Keywords: LI-6400XT, portable photosynthesis system, plant physiology, gas exchange, photosynthesis, respiration, transpiration, leaf chamber, IRGA, calibration, troubleshooting, A-Ci curve, CO2 response curve, practical, demonstration
Location:
Headquarter MPOB, Bangi
6, Persiaran Institut, Kawasan Institusi Bangi, 43000 Bandar Baru Bangi, Selangor
XP9R+9X Bandar Baru Bangi, Selangor
2.968770958080534, 101.7435232860253
Attribution 4.0 International — CC BY 4.0 - Creative Commons
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