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Physiology Lab Session Determination of Leaf Area Techniques (MARCH 2023)


Delivered on: 22 MARCH 2023


This video demonstrates three different methods for measuring leaf surface area, a crucial parameter in plant physiology and photosynthesis research. 🌿🔬 We'll explore the advantages and disadvantages of each technique and analyze the relationships between them.


Leaves are a plant's main photosynthetic organs. Leaf structure is closely associated with its photosynthetic function. Through the process called photosynthesis, plants absorb light through their leaves and use it to split water molecules into hydrogen and oxygen molecules. The oxygen is released into the atmosphere and the hydrogen is combined with carbon dioxide from the atmosphere to create sugar to feed the plant. It is clear that the plant's ability to create food is dependent on the surface area of its leaves. That is why the photosynthesis rate is directly related to the leaf surface area. To arrive at the rate of photosynthesis, therefore, you must calculate the leaf surface area of each plant, because most stomata are found in the lower epidermis, you will determine that surface area. There are a few methods to measure leaf surface area, such as:


a) Leaf surface area, by weight

By tracing the leaf area onto a piece of paper and cutting off the area traced. Weight the cut area. The weight can be related to the paper weight in which the area is known.


b) Count the number of squares on graph paper

To trace the leaf area on a piece of 1 cm grid of graph paper and count the total square cm on the graph.


c) Using an Automatic Leaf Area Meter (ALAM)

By using a device called the Automatic Leaf Area Meter (ALAM) that can be used to measure leaf surface area automatically. This method is easier and faster.


Objectives

1) To determine the leaf surface area by using these three methods above

2) To relate among methods of leaf surface area determination. The relationship is determined by plotting graphs and calculates the relationship statistically.


Keywords: Leaf surface area, photosynthesis, plant physiology, leaf morphology, measurement techniques, automatic leaf area meter (ALAM), graph paper method, weight method, science education, laboratory practical.


Location:

Faculty of Agriculture, Universiti Putra Malaysia

Fakulti Pertanian, Universiti Putra Malaysia, 43000 Seri Kembangan, Selangor

XPMM+9J Seri Kembangan, Selangor

2.9845517506267742, 101.73803356324866


Attribution 4.0 International — CC BY 4.0 - Creative Commons

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