Original video: https://youtu.be/js9tRn-Z86M
This video presents a fascinating look at the internal processes of adventitious root (AR) formation in microshoots, visualised using Magnetic Resonance Imaging (MRI). We compare the development of AR structures in two distinct rose genotypes: Herzogin Friederike (HF) and Mariatheresia (MT).
Adventitious root (AR) formation is one of the most important developmental processes in vegetative propagation. Although genotypic differences in rose rooting ability are well known, the causal factors are not well understood. The rooting of two contrasting genotypes, ‘Herzogin Friederike’ and ‘Mariatheresia’, was compared following a multiscale approach. Using magnetic resonance imaging, we non-invasively monitored the inner structure of stem cuttings during initiation and progression of AR formation for the first time. Spatially resolved Fourier-transform infrared spectroscopy characterized the chemical composition of the tissues involved in AR formation. The results were validated through light microscopy and complemented by immunolabelling. The outcome demonstrated similarity of both genotypes in root primordia formation, which did not result in root protrusion through the shoot cortex in the difficult-to-root genotype ‘Mariatheresia’. The biochemical composition of the contrasting genotypes highlighted main differences in cell wall-associated components. Further spectroscopic analysis of 15 contrasting rose genotypes confirmed the biochemical differences between easy- and difficult-to-root groups. Collectively, our data indicate that it is not the lack of root primordia limiting AR formation in these rose genotypes, but the firmness of the outer stem tissue and/or cell wall modifications that pose a mechanical barrier and prevent root extension and protrusion.
Citation:
David Wamhoff, André Gündel, Steffen Wagner, Stefan Ortleb, Ljudmilla Borisjuk, Traud Winkelmann, Anatomical limitations in adventitious root formation revealed by magnetic resonance imaging, infrared spectroscopy, and histology of rose genotypes with contrasting rooting phenotypes, Journal of Experimental Botany, Volume 75, Issue 16, 28 August 2024, Pages 4784–4801, https://doi.org/10.1093/jxb/erae158
Keywords: 3D imaging, Magnetic Resonance Imaging, Rosa hybrida, Plant Science
Published on: 12 April 2024
Attribution 4.0 International — CC BY 4.0 - Creative Commons
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