Original video: https://youtu.be/rscU8735deI
This video demonstrates the power of Fluorescence Recovery After Photobleaching (FRAP) for investigating the dynamic activity of xylem parenchyma cells in trees. We present a comparative study using ash (Fraxinus excelsior) and a hybrid poplar (Populus tremula × tremuloides), two species with contrasting wood anatomies and physiological strategies. FRAP experiments were performed on living transverse vibratome stem sections of ash (n=13) and poplar (n=13) incubated with FM 4-64FX at room temperature for 15 min and washed in distilled water. The protocol for FM 4-64FX intercellular transport in wood is based mostly on GAP-FRAP analyses (Abbaci et al., 2007; Stasiak et al., 2020).
Xylem parenchyma cells are living cells within the xylem tissue that play crucial roles in water and nutrient storage, transport regulation, defense responses, and recovery from embolism.
The video showcases FRAP experiments performed on wood samples collected during the spring and summer of 2019. Watch as a specific region within the xylem parenchyma is photobleached and then observe the subsequent recovery of fluorescence as unbleached molecules move into the bleached area.
Keywords: FRAP, Fluorescence Recovery After Photobleaching, Xylem Parenchyma, Xylem, Fraxinus excelsior, Ash, Populus tremula × tremuloides, Poplar, Hybrid Poplar, Plant Physiology
Citation:
Aleksandra Słupianek, Elżbieta Myśkow, Anna Kasprowicz-Maluśki, Alicja Dolzblasz, Roma Żytkowiak, Magdalena Turzańska, Katarzyna Sokołowska, Seasonal dynamics of cell-to-cell transport in angiosperm wood, Journal of Experimental Botany, Volume 75, Issue 5, 28 February 2024, Pages 1331–1346, https://doi.org/10.1093/jxb/erad469
Published on: 23 November 2023
Attribution 4.0 International — CC BY 4.0 - Creative Commons
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