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LICOR LI6800: Data Analysis at Forestry UPM Part 1 of 2


Delivered on 3 January 2024


This video is the first part of a workshop focused on analyzing data obtained from the LI-6800 Portable Photosynthesis System. Filmed at the Faculty of Forestry, Universiti Putra Malaysia (UPM), this session guides you through interpreting key parameters, understanding photosynthetic limitations, and extracting valuable insights from your measurements.


What you'll learn:

  • LI-6800 Data: We'll explore the various parameters measured by the LI-6800, including those related to photosynthesis, gas exchange, and chlorophyll fluorescence.

  • Steady-State Measurements: Understand the common variables measured during steady-state measurements and their significance in assessing photosynthetic performance.

  • Optimizing Measurement Conditions: Learn about the optimal time of day for taking measurements on C3 plants and the importance of considering factors like CO2 concentration, leaf temperature, and light intensity.

  • Data Interpretation: We'll guide you through interpreting key parameters like assimilation rate, stomatal conductance, intrinsic water use efficiency (iWUE), and instantaneous carboxylation efficiency (iCE)

  • Understanding Ca, Ci, and Cc: Explore the concepts of ambient CO2 (Ca), intercellular CO2 (Ci), and chloroplastic CO2 (Cc), and their relationship to photosynthesis.

  • Q&A Session: Engage in an interactive Q&A session to address specific questions and clarify concepts.


Video Timestamps:

00:00 Introduction Parameter in Excel file of the LI-6800

10:00 Common variable at steady state measurement

19:00 Optimum hour to take LI-6800 on C3 plant

24:20 Carbon dioxide CO2 location in plant

25:25 Ci Ca ratio

36:45 Leaf temperature, thermocouple

41:00 Light sensor, visible light PPFD

44:00 Avagadro's constant

53:00 Actinic light setting need blue light

01:01:01 Calculated variable

01:22:10 Data interpretation

01:54:00 Summary of data and improvement

01:59:00 Understanding Ca, Ci & CC

02:04:00 Fluorescene data

02:08:41 Checklist summary

02:21:00 Q&A

02:22:00 gsw vs transpiration


Keywords: LI-6800, photosynthesis, plant physiology, gas exchange, chlorophyll fluorescence, data analysis, stomatal conductance.


Watch Part 2 here: https://youtu.be/xcqnkm9fzlY


Location:

Faculty of Forestry and Environment

Persiaran Tulang Daing, 43400 Serdang, Selangor

2P35+32 Serdang, Selangor

3.0028342028498716, 101.70765826605694


Attribution 4.0 International — CC BY 4.0 - Creative Commons

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