Original video: https://youtu.be/Fy56p6mVyVY
Delivered on: 18DECEMBER2023
Learn how to precisely measure leaf dimensions using the free imageJ software! This tutorial demonstrates step-by-step how to:
Install and Set Up ImageJ: We'll guide you through installing the software and configuring it for accurate leaf measurements.
Import and Scale Images: Learn how to import leaf images and set the scale for accurate measurements in real-world units (e.g., cm).
Outline and Measure Leaf Area: Master the polygon selection tool to trace the outline of a leaf and calculate its total area.
Analyze Leaf Health: Discover how to use colour thresholding to isolate and measure healthy (green) and diseased or damaged areas of a leaf.
Calculate Leaf Parameters: We'll show you how to determine leaf length, width, and perimeter, along with the percentage of diseased leaf area.
Export Data: Learn how to efficiently export your measurements for further analysis and record-keeping.
Video Timestamps:
0:00- 0:50 Activity title
0:51- 1:00 Install ImageJ
1:05- 1:13 Select/ Drag the image into ImageJ.
1:50- 2:00 Use both hands to zoom or unzoom
2:01- 2:55 Set scale
2:56- 3:05 Double check with leaf width
3:06- 3:10 Press m on the keyboard to open measurement tab
3:11 - 7:30 Outline the whole leaf
7:31- 7:40 Clear outside
7:41 - 9:30 Measure leaf length, width and area
9:40- 11:10 Measure green area
11:11- 15:52 Transfer data to excel
Keywords: ImageJ, leaf measurement, leaf area, leaf perimeter, leaf health, plant science, botany, image analysis, tutorial, demonstration, free software.
Attribution 4.0 International — CC BY 4.0 - Creative Commons
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