Published on: 22 March 2023
Full paper: https://doi.org/10.1186/s12284-023-00629-0
Abstract
Tillering and yield are linked in rice, with significant efforts being invested to understand the genetic basis of this phenomenon. However, in addition to genetic factors, tillering is also influenced by the environment. Exploiting experiments in which seedlings were first grown in elevated CO2 (eCO2) before transfer and further growth under ambient CO2 (aCO2) levels, we found that even moderate exposure times to eCO2 were sufficient to induce tillering in seedlings, which was maintained in plants grown to maturity plants in controlled environment chambers. We then explored whether brief exposure to eCO2 (eCO2 priming) could be implemented to regulate tiller number and yield in the field. We designed a cost-effective growth system, using yeast to increase the CO2 level for the first 24 days of growth, and grew these seedlings to maturity in semi-field conditions in Malaysia. The increased growth caused by eCO2 priming translated into larger mature plants with increased tillering, panicle number, and improved grain filling and 1000 grain weight. In order to make the process more appealing to conventional rice farmers, we then developed a system in which fungal mycelium was used to generate the eCO2 via respiration of sugars derived by growing the fungus on lignocellulosic waste. Not only does this provide a sustainable source of CO2, it also has the added financial benefit to farmers of generating economically valuable oyster mushrooms as an end-product of mycelium growth. Our experiments show that the system is capable of generating sufficient CO2 to induce increased tillering in rice seedlings, leading eventually to 18% more tillers and panicles in mature paddy-grown crop. We discuss the potential of eCO2 priming as a rapidly implementable, broadly applicable and sustainable system to increase tillering, and thus yield potential in rice.
Keywords: Tiller, Yield, CO2, Climate, Rice
Background
Global annual increases in rice production are decreasing at a time of an increasing global population dependent on this crop as a staple food (Bin Rahman and Zhang 2022). This has led to an array of efforts to increase rice yield, both by breeding and implementing new agronomic approaches (Saito et al. 2021; Verma et al. 2021). Increased yields must be achieved in an environmentally and economically sustainable fashion—inputs to the agronomic system must be maintained at as low a level as feasible, and there must be economic incentives for rice farmers. For example, in Malaysia, the site of the experiments reported here, rice farmers represent an aging population whose income falls into the lower socio-economic bracket, despite the importance of rice nutritionally, socially and culturally to the wider society (Department of Statistics Malaysia 2021a, b).
With respect to rice yield, tiller number is closely linked to panicle number and, thus, the capacity of a plant to produce seed (Huang et al. 2020). Consequently, there have been numerous studies into the genetic basis for tillering and panicle development in rice (Saad 2014; Liang et al. 2014; Zhao et al. 2020; Huang et al. 2021), with significant advances in the identification of genetic traits controlling tiller number and inflorescence architecture. Studies have also provided insight into the complex network of internal signaling processes controlling tiller formation and bud development, including, for example, sucrose (Patil et al. 2022) and strigolactone (Fang et al. 2020). However, in addition to genetic factors, it is clear that environmental factors can also influence tiller development (Wang and Li 2005; Assuero and Tognetti 2010; Saad 2014). These include the circadian clock (Wang et al. 2020), temperature (Xu et al. 2020), CO2 (Seneweera 2011; Zhou et al. 2021; Liu et al. 2021) and the availability of nutrients (Wang et al. 2017).
With respect to CO2, a meta-analysis of 20 years of rice Free Air CO2 Enrichment studies, showed that panicle number per ha, spikelet number per panicle, fully-filled grain percentage and grain weight were all significantly increased in plants grown at high levels of CO2 (500–645 ppm) throughout their lifetime (Hu et al. 2021). To what extent these results reflected a general increase in growth linked to prolonged exposure to CO2 or whether there was a more specific developmental response to elevated CO2 remains unclear. For example, Jitla et al. (1997) showed that exposure to high levels of CO2 increased the number of tillers in mature Oryza sativa L., and that rice plants can respond to elevated CO2 at an early stage of development, but further analysis of this response has been limited. It is interesting that some leaf traits, such as stomatal density, can be set extremely early in leaf development by CO2 level, with the phenotype of the mature leaf set during this early phase (Lake et al. 2001). It is unknown whether other aspects of plant structure such as tiller formation display a similar regulation, i.e., at what point in rice plant development is tiller number set?
In this paper, we first describe a series of experiments designed to explore when rice plants can respond to CO2 in terms of increased tillering. We then explore whether the observed response can be exploited to induce tillering and yield in semi-field and field conditions in Malaysia, a hub of rice production in S.E. Asia.
Materials and Methods
Plant Material and Growth Conditions
Oryza sativa (Indica-IR64) seeds were provided by the International Rice Research institute. Oryza sativa (Indica-MR219) and Oryza sativa (Indica-MR263) seeds were provided by the Genebank and Seed Centre, Malaysian Agricultural Research and Development Institute (MARDI).
Commercial Controlled Environment Chambers
Experiments were carried out in a set of paired plant growth chambers (Conviron PGR15; Conviron, Winnipeg, Canada) at 70% relative humidity, in a 12/12 h light/dark cycle at 28/24 °C with a light intensity of 750 μmol m⁻² s⁻¹ at canopy height. CO2 was either kept at ambient levels (430 ppm) with no added CO2 (aCO2 chamber) or CO2 was controlled at 800 ppm (eCO2 chamber). Seeds were germinated on filter paper with 15 ml water in petri dishes, then grown in 13D pots (0.88L) filled with 71% Kettering Loam (Boughton, UK), 23.5% Vitax John Innes No. 3 (Leicester, UK), 5% silica sand and 0.5% Osmocote Extract Standard 5–6 month slow-release fertilizer (ICL, Ipswich, UK) by volume, saturated with water. aCO2 plants and eCO2 plants were germinated and grown exclusively in the aCO2 chamber or eCO2 chamber respectively. eCO2 primed plants were germinated and grown in the eCO2 chamber for the number of days indicated, then transferred to the aCO2 chamber. Ten plants were used for tillering analysis except for the aCO2 treatment of MR219 where n = 9.
Yeast-Controlled Propagators—Semi-Field Trial
Propagators were constructed as shown in Additional file 1: Fig. S1A. Temperature was controlled at 24–30 °C, with a 12 h/12 h light/dark cycle. Light was provided by 12 units of 30 watts, 1.2 m T8 LED growth light with a red:blue LED ratio of 3:1, and one unit of white 16-W T5 LED. Relative humidity for aCO2 was 72–76% (day) and 73–83% (night), and for eCO2 78–80% (day) and 80–86% (night). A mix of 600 g sugar, 1.5 L distilled water, and a packet of 11 g baker's yeast (Saccharomyces cerevisiae) in a 2.7 L plastic bottle was used to generate eCO2, with detailed measurements of CO2 shown in Additional file 1: Fig. S1B. Each fermentation mix lasted 10 days before being replaced. Seeds were soaked in distilled water for 24 h, then germinated in the aCO2 or eCO2 propagators on wet paper towel in a petri dish for 2–3 days, then three seedlings were transferred to each 8 fl oz container with standard soil media mixture (3:2:1 topsoil:sand:burned rice husk), and submerged in 2–3 cm of water. Seedlings were fertilized at day 5 and 15 with 1 g NPK 15:15:15 and 1 g urea per pot. 25 days after sowing (DAS), seedlings were transplanted two seedlings to a 1 gallon container filled with soil taken from a paddy field in Tanjong Karang, Selangor and transferred to semi-field conditions (a rainout shelter structure at Field 15 research farm, Universiti Putra Malaysia, Serdang) from October 2018 to March 2019. Plants were drip irrigated. Air temperature was 25–38 °C, humidity level was 60–80% and ambient CO2 ranged between 374 and 434 ppm. The fertilisation schedule was as in (Saad 2014). Wood vinegar and neem oil-based solution was applied every two weeks, and solar-powered ultrasonic animal repellent (OEM, China) were used to control pest infestations.
Mycelium-Controlled Chambers—Paddy Trial
The chambers each contained a full spectrum LED light panel (Samsung LM281B AC 100–265 V, Shenzhen Colighting Ltd., China) and a six-inch exhaust fan (Additional file 2: Fig. S2A). A light/dark 11/13 h schedule was used, with light intensity measured using a spectrometer (LI-180; LI-COR Inc., Lincoln, NE, USA) at 740 μmol m⁻² s⁻¹ PPFD (photosynthetic photon flux density) (Blue mean intensity: 119 μmol mm⁻² s⁻¹; Red mean intensity 308 μmol m⁻² s⁻¹). Oyster mushroom (Pleurotus pulmonarius (Fr.) Quel) mycelium bags used a substrate ratio of 100:10:1 sawdust:rice bran:CaCO3 powder and water (60–70% of sawdust weight) from Nas Agro Mushroom Farm, Malaysia. The eCO2 chamber contained 34 one-week injected mycelium bags, covered with a damp cloth. CO2 levels are shown in Additional file 2: Fig. S2B. Seeds were germinated within the aCO2 and eCO2 chambers for 3 days, then transplanted into a 12 pod tray (19 cm × 14.5 cm × 11 cm) filled with saturated peat moss:neem cake 10:1 and covered with a plastic propagator lid. Seedlings were thinned at 7 DAS to one seedling per pod. Neem oil (0.5% v/v) was sprayed at 14 DAS, 21 DAS and 28 DAS. The watering solution was changed to AB fertiliser solution with a concentration of 1800–2000 µS/cm at 8 DAS and was replaced every two days. At 30 DAS, seedlings were transplanted into 6 × 0.9 m² irrigated plots, with a spacing of 30 cm between plants. The paddy soil was a silty clay texture (0.80% sand, 54.20% silt, and 45.10% clay) with pH 4.74 and 1.30% organic carbon. Plants were grown in the paddy field at the MARDI Field Station, Parit, Perak, from April-July 2022. Irrigation was maintained at 5–10 cm above the ground until 95 DAS. Insecticides (Karate—Syngenta, Match 050 EC—Syngenta, Nurelle D505 EC—Dow Agrosciences, Prevathon 5SC—DuPont, Pexalon—Dupont, Alika ZC—Syngenta), herbicides (Sofit 300 EC—Syngenta, Loyant—Dow Agrosciences), fungicide (Stinger—DuPont), and fertilisers (NPK Blue (12:12:17:2TE) and urea) were used following the typical schedules used at the MARDI Field Station.
Plant Growth and Yield Analysis
A tiller was defined as any stem with more than one leaf—the main culm was not included in counts. The basal 5 cm of rice plants were prepared for tiller base images by fixing in 1:4 Acetic anhydride:EtOH for two weeks. The sample was cut to 1 cm above the base of the shoot and imaged from above on a LEICA M165 FC Stereomicroscope and built in LEICA DFC 450 C camera. Developing tillers were marked and counted.
Seedling measurements were taken at 24DAS for the yeast-controlled propagators, and 28DAS for the mycelium-controlled chamber. Measurement of plant height was taken from the surface of the soil to the highest shoot tip. Leaf number was manually counted on the fully expanded leaves. For plant height and leaf number measurements, each point is the mean of three seedlings. For dry weights, plants were separated into shoot and root, then dried for 48–72 h at 60C before weighing.
Sixteen plants were used in all semi-field yield analyses, except grain filling where a sample of 10 plants were used. Paddy-field yield analyses were performed on 29 or 30 plants, as indicated. Yield was analysed at 141 DAS (semi-field conditions) and 127 DAS (paddy conditions). Tillers and panicles were counted manually. Leaf biomass was dried as for seedling dry weights. Dried filled grains and unfilled and partially filled grains were counted. A complete set of 16 spikelets from 16 (semi-field experiment) or 29/30 (paddy-field experiment) were counted for 1000 grain weight using a seed counter (TRILITE, China). Percentage of filled seeds was calculated.
Semi-field yield was calculated as:
Paddy field yield was calculated as:
Statistical Analysis
All statistical analysis was performed in Graphpad Prism versus 9.3.1.
Results
eCO2 Priming in Controlled Environment Chambers Leads to Increased Tillering in Rice
Rice plants grown constantly under elevated CO2 (eCO2) conditions showed an increased number of tillers (Fig. 1A, B). To identify when this increase was set, we grew young plants at high levels of CO2for defined periods before transferring the plants to ambient CO2 levels, then measuring tiller number at plant maturity. Thus, rice plants (IR64) were primed at high CO2 for the first 21, 28 or 35 days after sowing (DAS) and then transferred to ambient CO2 (aCO2) conditions until they reached vegetative maturity (Fig. 1A, B). Control plants were maintained at aCO2 and eCO2 for the duration of the experiment. Plants which were eCO2 primed for 21 days were indistinguishable from aCO2 controls, however, plants eCO2 primed for 28 or 35 days had an increased number of tillers, comparable to the eCO2 controls at 49 DAS.
Fig.1
To better understand this increased tillering response, and to see if the effect was replicable with different cultivars, seedlings of IR64 and two cultivars of elite rice grown in Malaysia (MR219 and MR263) were eCO2 primed for 28 days then transferred to aCO2 conditions. These were compared to plants grown only under aCO2 conditions. Tillers were counted weekly from sowing to 57 DAS (Fig. 1C–H). All three cultivars showed a significantly increased number of tillers by 14 DAS. In IR64 and MR263 plants, the difference in tiller number remained significant until the end of the growth period (57 DAS) (unpaired t tests, * p < 0.05, ** p < 0.01, *** p < 0.005, n = 9/10) (Fig. 1C, E). Individual data points are shown from 43 DAS in Fig. 1F, H. MR219 plants had a higher mean number of tillers throughout the growth period, but this difference was not significant after 28 DAS (Fig. 1D, G). Plants grown in the same conditions were destructively sampled at 28 DAS to show the increased number of tillers at the base of the plant when grown at eCO2 (Additional file 3: Fig. S3).
Yeast Driven eCO2 Priming Increases Seedling Growth and Final Yield
The results shown in Fig. 1 indicated that exposure of rice seedlings to eCO2 for 28 days led to increased tillering at maturity, a factor expected to lead to increased crop yield in the field. This time period is similar to that used in traditional rice cultivation where seedlings are first grown in nursery plots prior to transfer to the paddy. To explore whether our observations based on controlled environment chambers could be of potential value in an agronomic setting, we set out to replicate the CO2 priming treatment in a rice growing area in Malaysia.
To do this, we first developed an economic in-house growth propagator system capable of creating elevated CO2 conditions using a combination of yeast, water and sugar to generate CO2 (Additional file 1: Fig. S1A). CO2 levels within the eCO2 propagator were on average 1000 ppm during the day and between 1500 and 2000 ppm at night, while the aCO2 propagator had reasonably constant CO2 levels of between 390 and 420 ppm (Additional file 1: Fig. S1A). We could thus generate a CO2-enriched environment for seedling growth. MR219 seedlings were grown in the eCO2 propagator for 24 days (Fig. 2A) and compared with plants grown for the same time in a propagator under aCO2 conditions (Fig. 2B). eCO2 grown plants were significantly taller, with an average of 42% increase in height compared with aCO2 controls (Fig. 2C, unpaired t test, p < 0.0001, n = 7). Seedlings grown at eCO2 also had more leaves than aCO2 controls (Fig. 2D, unpaired t test, p < 0.0001, n = 7), a 31% increase in shoot weight, and a 160% increase in root weight (Fig. 2E, F, unpaired t test, p = 0.0107 and p = 0.0002, n = 7). Thus, growing rice seedlings in the eCO2 propagator in Malaysia for a short time led to a promotion of growth.
Fig.2
To investigate the outcome of elevated CO2 during the seedling stage on final plant yield, a semi-field trial was performed in which eCO2 primed and aCO2 seedlings were transplanted into 1-gallon containers (two plants per container) and moved into a field-based rain shelter in a random block design until maturity (Fig. 3A). At 141 DAS, plants were harvested, and various parameters were measured (Fig. 3B–F). Plants which had been eCO2 primed in the yeast propagator for 25 days had more tillers (Fig. 3B) and panicles (Fig. 3C) than those grown at aCO2 for the duration of their growth. Analysis of yield on ten plants per treatment revealed that the number of filled grains per panicle was improved in eCO2 primed plants (Fig. 3D, unpaired t test, p = 0.0019, n = 10) as was the percentage of filled seeds (Fig. 3E, p = 0.0003, n = 10). Thousand grain weight was also increased in the eCO2 treated plants compared with the controls (Fig. 4F, unpaired t test, p = 0.0037, n = 16). Based on these data, an average yield per plant was calculated as 36.6 g for eCO2 primed plants, and 28.4 g for aCO2 control plants, equivalent to an estimated yield increase of 29%.
Fig.3
Fig.4
Mycelium Generated eCO2 Priming Increases Plant Growth in Paddy Conditions
Encouraged by the semi-field experiments, we adapted the CO2 priming system to make it more applicable to the field. In order to find a system that not only elevated local CO2, but also provided a potential extra revenue source for farmers, we investigated using bags of Oyster mushroom fungal mycelium rather than yeast as a CO2 source. The chamber design was also improved to increase ease of use (Additional file 2: Fig. S2A). Oyster mushrooms were chosen since the fruiting bodies have relatively high economic value. Under our growth conditions, mycelium bags produced fruiting bodies after 28–35 days, with a second bloom after a further 20–25 days. In addition, the compost generated by mycelium breakdown has a market value for horticulture. Finally, the substrate used to grow the fungus was agricultural lignocellulosic waste, which is both plentiful and low-cost in Malaysia. Under our conditions, the mycelium-derived CO2 showed a diurnal cycle, with an average CO2 level of 655 ppm during the day and 1540 ppm at night in the eCO2 chamber. In contrast the aCO2 chamber showed a fairly constant level of 450 ppm CO2 (Additional file 2: Fig. S2B).
The effect of 28 days of mycelium-driven eCO2 priming on rice seedlings was similar to that observed in plants grown in the yeast eCO2 propagator (Fig. 4A, B). eCO2 primed seedlings were taller than aCO2 controls (Fig. 4C, unpaired t test with Welch’s correction, p < 0.0001, n = 20) and had a slightly increased number of tillers (Fig. 4D, Mann–Whitney U test, p = 0.0519, n = 20). There was an increased shoot weight (Fig. 4E, unpaired t test with Welch’s correction, p < 0.0001, n = 20) and root weight (Fig. 4E, unpaired t test, p < 0.0001, n = 20) in the eCO2 plants.
After 28 days in the mycelium eCO2 chambers, seedlings were transplanted to paddy conditions (Fig. 5A, B) and grown to maturity (Fig. 5C, D). In a similar manner to the semi-field trial, paddy grown plants after eCO2 priming were larger at harvest (127 DAS), with a greater final leaf blade biomass compared to aCO2 controls (Fig. 5E, Mann–Whitney U test, p = 0.0118, n = 29/30). There was an increased number of tillers (Fig. 5F, unpaired t tests, p = 0.0197, n = 29/30) and more panicles per plant (Fig. 5G, Mann–Whitney U test, p = 0.0118, n = 29/30) than controls grown in aCO2 chambers for the first 28 days. However, unlike the rain shelter experiment, these positive outcomes on tiller and panicle number did not translate into an increase in yield in the eCO2 primed plants, with no significant differences in number of filled grains per panicle (Fig. 5H, percentage of seeds which were filled (Fig. 5I), indeed these values tended to be smaller in the eCO2-treated plants. This compensation for increased tiller and panicle number resulted in overall estimated yield per ha being similar for both treatments (Fig. 5J, unpaired t tests, p = 0.1118, n = 29/30).
Fig.5
Discussion
Increasing rice yield in a sustainable fashion is a major global challenge. The majority of efforts in this research area have focused on genetic improvements in a wide variety of traits, including structural features linked to yield (Xing and Zhang 2010; Siddiq and Vemireddy 2021). In this context, tillering has been of major interest. The number of tillers formed by a plant will set the maximum number of potential inflorescences, the maximum number of panicles, and thus the theoretical maximum number of seeds generated by a single plant. Whether this potential is reached will, of course, be dependent on a host of factors—notably the source capacity of the plant and the portion of resources transported to the developing seed (Chang and Zhu 2017). The results reported here provide an insight into the control of tillering in rice by an environmental signal, elevated CO2 level (eCO2). In particular, our work has shown that exposing rice seedlings to eCO2 for the first 24–28 days after germination promotes tillering in three different rice varieties and that, moreover, this increase in tiller number is generally maintained when the plants are transferred to ambient CO2 (aCO2) conditions.
Tillers are formed by a branching mechanism in the shoot apical meristem and the associated axillary meristems (Li et al. 2003, 2018; Wang and Li 2011). The rice embryo already has about three leaves, each with a dormant axillary meristem (Itoh et al. 2000). Each new leaf formed after germination has its own axillary meristem, thus already at a very early stage of growth rice seedlings have the potential to grow tillers. Under normal conditions, the potential for these meristems to form tillers is only revealed relatively late in the growth cycle as axillary bud growth is activated (Wang and Li 2011). The increased tillering observed in plants grown in eCO2 could reflect either a general promotion of growth via eCO2 which, as a by-product, leads to earlier release of axillary meristems to form tillers, or it could be a more direct action of eCO2 on the activation of axillary growth.
After a short eCO2 priming period an increase in tillers is clearly visible in sections across the base of our rice plants (Additional file 3: Fig. S3) and seedling growth is increased, as demonstrated by increased biomass. We therefore tend towards interpreting any increased tillering as a more indirect outcome of CO2 on growth, but further work analyzing exactly when and where axillary meristems are activated in the eCO2 plants would help clarify this question. It is also interesting to note that there was an element of cultivar-specificity in the degree of response to eCO2 priming with respect to tiller response. Again, whether this reflects differences in general plant growth response to elevated CO2, or whether there is an element of specificity in tillering response to CO2 awaits warrants further investigation.
Although there has been an increasing push towards modernising rice agronomy via, e.g., direct planting (Kumar and Ladha 2011; Jat et al. 2022; Su et al. 2022), much current rice production remains dependent upon traditional approaches in which rice seedlings are initially grown in nurseries prior to transplanting to the paddy. There is thus a time during early growth (3–4 weeks) when the entire rice crop is essentially concentrated in a small area. We explored the idea that exposing rice plants to eCO2 for this short phase of crop growth might lead to a similar phenotype of increased growth and tillering to increase the rice yield potential in the mature crop. By transplanting eCO2 primed seedlings to field or semi-field conditions, we investigated the outcome on actual crop yield.
The first challenge was to devise a method of providing elevated CO2 to rice seedlings outside of conventional controlled environment chambers. To do this we explored two routes: fermentation of sugars via baker’s yeast and respiration via fungal mycelium growing on lignocellulosic waste. Both approaches proved effective at elevating local CO2 concentrations, generating CO2 levels in excess of 1000 ppm. Yeast fermentation provided a more consistent level of eCO2, whereas fungal respiration displayed a strong diurnal rhythm, with high levels achieved during the night and levels falling towards (but not reaching) aCO2 levels during the day (suggesting that the CO2 effect may not be via photosynthesis). Despite these differences in eCO2 profile, a comparable response in terms of increased tillering and growth was observed. These data suggest that the response does not require a steady high level of CO2 and fit more to an interpretation of integrated elevated CO2 over a time period of a few weeks being sufficient to elicit a response of increased tillering and growth in rice seedlings. These data are consistent with previous observations made by Jitla et al. (1997) who reported an increase in seedling tillering and growth rate when germinated in high CO2, and a higher rice tiller number and grain yield (g plant−1) in controlled growth chambers provided with elevated CO2 from 15 days after sowing. Our data show that this early promotion of tillering in responsive cultivars is maintained as the plants grow to maturity under ambient CO2 level, both in field and semi-field conditions. A difference was observed, however, between our field and semi-field experiments in the extent to which the theoretical increase in yield capacity was actually translated into yield.
In semi-field conditions, with plants transferred to large containers under a rain shelter after eCO2 priming, we saw a significant increase in final yield. A higher number of filled grains per panicle, a higher percentage of seeds filled, and improved grain size (1000 grain weight) drove this increase. In contrast, although the plants grown to maturity in paddy field conditions after mycelium eCO2 priming were significantly larger than the controls, with an increased number of tillers, panicles and leaf blade biomass, this did not translate into increased yield. Notably, the proportion of seeds which were filled was less than control, i.e. the capacity for increased yield was there but not exploited by the plants.
The reasons for this are potentially complex. First of all, the plants were grown using standard agronomic practice in the region, using normal recommended supplements of N and P. It is thus possible that although the treatment led to increased sink potential, the source activity was insufficient to exploit this potential. Supplying increased fertilizer might remedy this, but obviously would lead to extra costs (economic and environmental). It should also be noted that our exploratory field experiments were heavily impacted by the recent pandemic, with access to field sites being intermittently highly restricted. Consequently, the paddy field trial was performed towards the end of the standard growing period, leading to a noticeably high level of insect pest damage. It is also worth noting that growth in elevated CO2 has been reported to inhibit secondary metabolism, thus limiting crop resistance to attack (Bazinet et al. 2022). Thus, there are biotic and abiotic factors which are likely to have impacted the final stage of crop growth. Now that field access is fully open post-pandemic, repeating the field experiment with or without increased nutrient supply would be of interest. The data from our semi-field experiments clearly indicate that the increased sink capacity via increased tillering and panicle formation after CO2 priming can be exploited by the rice plants, but achievement of this potential in the field may require increased inputs to the system.
For the exploratory field experiments, we investigated using fungal mycelium to generate CO2 to promote early rice growth. The reasoning behind this was to explore whether the use of commercially important fungi (oyster mushrooms) might provide farmers with an added income stream, thus adding to economic benefits derived from increased rice yield. In addition, the fungus was grown on lignocellulosic waste products which are plentiful and inexpensive in Malaysia. The fungus yields two harvests of fruiting bodies during the priming process, which provides an additional potential income. Moreover, the hyphae/cellulosic waste after priming can be sold as a product in the form of compost. Obviously, there are costs associated with building and running the chambers used in this study to prime the rice seedlings, so capital expenditure is required to implement the system. Further research to explore the use of eCO2 priming systems in field conditions would enable a better assessment of the application of the process in a range of rice-growing areas with different agronomic challenges and economic conditions.
Conclusion
In conclusion, this work identifies an environmental trigger (CO2) which can be used to manipulate tiller formation in a range of rice cultivars. We propose and demonstrate a mechanism by which this finding can be translated to the field situation to increase yield potential, an approach which may be of interest to a wide spectrum of growers using traditional methods to grow rice.
Availability of Data and Materials
The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
Abbreviations
aCO2:
Ambient CO2
eCO2:
Elevated CO2
MARDI:
Malaysian Agricultural Research and Development Institute
DAS:
Days after sowing
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Acknowledgements
Thank you to Christopher Hepworth and David Rapley for preliminary discussions on eCO2 priming. Open access was funded by the University of Sheffield Institutional Open Access Fund.
Funding
This project was supported by a Royal Society GCRF Challenge-Led grant CHL\R1\18007 “Breeding Rice Resilient to a High CO2 Future” awarded to N.Y., X-G. Z. and AJF.
Author information
Author notes
J. M. Sloan, A. A. M. Mujab and J. Mashitah contributed equally to this work
Authors and Affiliations
School of Biosciences, Plants, Photosynthesis and Soil, The University of Sheffield, Western Bank, Sheffield, S10 2TN, UK
Jennifer M. Sloan, Matthew J. Wilson & Andrew J. Fleming
Commercialization and Business Centre, Malaysian Agricultural Research and Development Institute, MARDI Parit, 32800, Parit, Perak, Malaysia
Azzami Adam Muhamad Mujab
Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, UPM, 43400, Serdang, Selangor, Malaysia
Azzami Adam Muhamad Mujab, Jusoh Mashitah, Liang Su Toh, A. Jalil Nur Zahirah, Kamaruzali Afiq, Ahmad Tajuddin Asyraf & Nazmin Yaapar
Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, UPM, 43400, Serdang, Selangor, Malaysia
Berahim Zulkarami
Center of Excellence for Molecular Plant Science, Institute of Plant Physiology and Ecology, CAS, Shanghai, 200032, China
Xin-Guang Zhu
Contributions
JS, AAMM, MJ, BZ, MJW, TLS, AJNZ, KA and AAT performed the experiments; JS, AAMM, MJ, X-GZ, NY and AJF interpreted the results and wrote the paper, with contributions from all authors. AJF and NY designed the study and led the project. All authors read and approved the final manuscript.
Corresponding authors
Correspondence to Nazmin Yaapar or Andrew J. Fleming
Supplementary Information
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