Original video: https://youtu.be/mUoA_qwV2vg
Delivered on: 23 FEBRUARY 2023
This comprehensive video explores the fundamentals of plant physiology, taking you from the basic needs of plants to advanced techniques using the LI-6800 Photosynthesis System to measure chlorophyll fluorescence and generate light response curves. 🌿🔬
Slot 1: Five Basic Needs of Plants and Experimental Layout
Air: Temperature, Humidity, CO2 and O2 concentration
Light: Spectrum, Intensity, Photoperiod
Water: Temperature, pH, EC, Oxygen content
Nutrients: Composition, Purity
Growing Medium: Air content, Moisture content
General Experimental Design
Slot 2: Chloroplast Under Light: Photosystems and Fluorescence
Light-dependent reactions: PS II, pigments, electron transport, ATP and NADPH production
Electromagnetic Spectrum and Visible Light
Cyclic and noncyclic photophosphorylation in photosystems
Photobiology: Photosynthesis and Fluorescence
Measured parameters: Fo, Fm, F, Fm', and Fo'
Light Response Curve: Theory and Components
Slot 3: Measuring Chlorophyll Fluorescence for Fv/Fm (maximum quantum efficiency of PSII reaction centers)
LI-6800 components and setting-up
Components of equipment and functions
Plants preparations and dark acclimation
Slot 4: Light Response Curve with Prior-Continuous Fv/Fm
Photo acclimation of leave to the highest light intensity
AutoProg: Performing Light response curve
Slot 5: Data Extractions and Interpretation
Interpreting Fv/Fm for maximum PSII efficiency
Interpreting light curve for:
Dark respiration rate: The photosynthesis rate with no light,
Light compensation point: Absorbed quantum flux for which photosynthesis and respiration are balanced.
Quantum efficiency: Initial slope of the assimilation rate, and
Light saturated photosynthetic rate (Asat).
Keywords: plant requirements, experimental design, biometrics, CRD, RCBD, split plot, nested design, experimental unit, observational unit, confounding effects, photosynthesis, light reactions, synthesis reactions, Calvin cycle, chlorophyll fluorescence, FvFm, PSII efficiency, light response curve, quantum yield, photoinhibition, CO2 assimilation, electron transport rate, light intensity, light quality, light duration, humidity, temperature, nutrient management, hydroponics, plant growth regulators
Location:
Faculty of Agriculture, Universiti Putra Malaysia
Fakulti Pertanian, Universiti Putra Malaysia, 43000 Seri Kembangan, Selangor
XPMM+9J Seri Kembangan, Selangor
2.9845517506267742, 101.73803356324866
Attribution 4.0 International — CC BY 4.0 - Creative Commons
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