Original video: https://youtu.be/01xs6bYt8SY
Delivered on: 20 May 2024
This video explores two key plant hormones: Auxin and Gibberellic Acid (GA3), used in a laboratory practical at Universiti Putra Malaysia.
Auxin, also known as indole-3 acetic acid (IAA), is a well-studied plant hormone that is responsible for plant growth in response to biotic and abiotic stresses. Usually, synthetic auxins like α-Naphthaleneacetic acid (αNAA) and 2,4-Dichlorophenoxyacetic acid (2,4-D) are used as herbicides. They have been in use as herbicides for the past fifty years due to their high effectiveness and cheap cost. Adequate production of the plant hormone auxin or indole-3-acetic acid (IAA) can efficiently promote plant root growth. Although auxin is a growth promoting hormone, it can be a metabolic burden to plants at high concentrations and therefore toxic. Synthetic auxins are extremely stable and can persist in the soil for weeks, which is why they are extremely effective herbicides. IAA, on the other hand, is chemically labile and can be easily metabolized by the plants.
The uses of synthetic auxins in horticulture can be traced directly to the natural roles of IAA in the plant. In general, compounds such as a-naphthalene acetic acid (NAA) are used because they resemble IAA in action but are resistant to degradation by plant enzymes. Auxins are used for a variety of agricultural purposes, including: promotion of rooting of cuttings. The base of the cutting is dipped in a powder containing NAA or indolebutyric acid (IBA) prior to planting. Root initiation tests are best known for their role in evaluating materials and cultural techniques for plant propagation purposes. In the laboratory, these tests have been used in studies of the growth and elaboration of plant parts, and have aided the characterization and identification of naturally occurring growth-substances.
Gibberellic Acid-3 (GA-3) is a naturally occurring plant growth regulator which may cause a variety of effects, including the stimulation of seed germination in some cases. GA-3 occurs naturally in the seeds of many species and is produced commercially by growing Gibberella fujikuroi fungus cultures in vats, then extracting and purifying the GA-3. Presoaking seeds in GA-3 solution will in many cases cause the rapid germination of many types of highly dormant seeds which would otherwise need cold treatment, after-ripening or ageing, or other prolonged pretreatments. Many different types of dormancy are overcome with GA-3. Not all seeds respond well. A great deal of research needs to be done to determine which species benefit, and the proper concentration of GA-3 for each type.
Keywords: Practical, Plant Physiology, Auxin, Gibberellic Acid, Root Initiation, Seed Germination, Plant Growth, Plant Development, Plant Science, Agriculture, Botany, Laboratory Techniques, Scientific Experiment, Education, Science Education
Location:
Faculty of Agriculture, Universiti Putra Malaysia
Fakulti Pertanian, Universiti Putra Malaysia, 43000 Seri Kembangan, Selangor
XPMM+9J Seri Kembangan, Selangor
2.9845517506267742, 101.73803356324866
Attribution 4.0 International — CC BY 4.0 - Creative Commons
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