3D Animation: Arabidopsis Root Meristem Morphology and Cellular Segmentation

Original video: https://youtu.be/Vsu5dfnYZ2g MorphoGraphX28 was deployed to precisely quantify the 3D geometry of meristematic cells in WT and BR-signalling-perturbed roots and to compare the length, width, depth, surface area and volume of various tissues. This video presents a 3D animation visualising the intricate root meristem morphology and cellular segmentation of Arabidopsis thaliana. The animation showcases the dynamic structure of the root tip.

Visual Breakdown:

1. Greyscale: Raw confocal microscopy stack showing the cell wall signal.

2. Arbitrary Colors: 3D cellular segmentation with individual cells colored randomly.

3. Cell Type Colors: 3D cellular segmentation with cell types identified by specific colors:

• Green: Epidermis (dark green for hair cells, light green for non-hair cells)

• Blue: Cortex

• Yellow: Endodermis

• Pink: Pericycle

• Cyan: Stele

Explore the detailed organization of the Arabidopsis root meristem in this visually informative animation.

Keywords: Arabidopsis, Root Meristem, Morphology, Cellular Segmentation, 3D, Microscopy

Citation:

Fridman, Y., Strauss, S., Horev, G. et al. The root meristem is shaped by brassinosteroid control of cell geometry. Nat. Plants 7, 1475–1484 (2021). https://doi.org/10.1038/s41477-021-01014-9

Published: 15 November 2021

Attribution 4.0 International — CC BY 4.0 - Creative Commons